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Cusabio p cdc25
P Cdc25, supplied by Cusabio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+cdc25/Rabbit+anti-+Phospho-CDC25C+Polyclonal+Antibody/pm37752684-64-57-62
Average 91 stars, based on 1 article reviews
p cdc25 - by Bioz Stars, 2026-09
91/100 stars

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Related Articles

Blocking Assay:

Article Title: HOXD8 suppresses renal cell carcinoma growth by upregulating SHMT1 expression.
Article Snippet: Then, proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. After blocking with 5% skim milk, the membranes were incubated with primary antibodies against SHMT1 (1:1000, D3B3J, CST), cyclinB (1:1000, 4138S, CST), CDC20 (1:1000, 4823S, CST), β- actin (1:1000, A00702, GenScript), HOXD8 (1:1000, sc- 515357, SantaCruz), p21 (1:1000, A1483, ABclonal), γ- H2AX (1:500, CSB- PA010097OA139phHU, CUSABIO), p- CDK1 (1:1000, CSB- PA000492, CUSABIO), pCHK1 (1:1000, CSB- PA006769, CUSABIO), p- CDC25 (1:1000, CSB- PA080107, CUSABIO), cyclinE (1:1000, sc- 377100, SANTA), and cyclinD (1:1000, WL01435a, WanleiBio) overnight at 4°C with shaking. .. Then, the membranes were washed with Tris Buffered Saline with Tween20 (TBST) and incubated with secondary antibody conjugated with HRP (1:5000, GenScript) for 1 h at room temperature.

Article Title: HOXD8 suppresses renal cell carcinoma growth by upregulating SHMT1 expression
Article Snippet: Then, proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. After blocking with 5% skim milk, the membranes were incubated with primary antibodies against SHMT1 (1:1000, D3B3J, CST), cyclinB (1:1000, 4138S, CST), CDC20 (1:1000, 4823S, CST), β‐actin (1:1000, A00702, GenScript), HOXD8 (1:1000, sc‐515357, SantaCruz), p21 (1:1000, A1483, ABclonal), γ‐H2AX (1:500, CSB‐PA010097OA139phHU, CUSABIO), p‐CDK1 (1:1000, CSB‐PA000492, CUSABIO), p‐CHK1 (1:1000, CSB‐PA006769, CUSABIO), p‐CDC25 (1:1000, CSB‐PA080107, CUSABIO), cyclinE (1:1000, sc‐377100, SANTA), and cyclinD (1:1000, WL01435a, WanleiBio) overnight at 4°C with shaking. .. Then, the membranes were washed with Tris Buffered Saline with Tween20 (TBST) and incubated with secondary antibody conjugated with HRP (1:5000, GenScript) for 1 h at room temperature.

Incubation:

Article Title: HOXD8 suppresses renal cell carcinoma growth by upregulating SHMT1 expression.
Article Snippet: Then, proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. After blocking with 5% skim milk, the membranes were incubated with primary antibodies against SHMT1 (1:1000, D3B3J, CST), cyclinB (1:1000, 4138S, CST), CDC20 (1:1000, 4823S, CST), β- actin (1:1000, A00702, GenScript), HOXD8 (1:1000, sc- 515357, SantaCruz), p21 (1:1000, A1483, ABclonal), γ- H2AX (1:500, CSB- PA010097OA139phHU, CUSABIO), p- CDK1 (1:1000, CSB- PA000492, CUSABIO), pCHK1 (1:1000, CSB- PA006769, CUSABIO), p- CDC25 (1:1000, CSB- PA080107, CUSABIO), cyclinE (1:1000, sc- 377100, SANTA), and cyclinD (1:1000, WL01435a, WanleiBio) overnight at 4°C with shaking. .. Then, the membranes were washed with Tris Buffered Saline with Tween20 (TBST) and incubated with secondary antibody conjugated with HRP (1:5000, GenScript) for 1 h at room temperature.

Article Title: HOXD8 suppresses renal cell carcinoma growth by upregulating SHMT1 expression
Article Snippet: Then, proteins were transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore). .. After blocking with 5% skim milk, the membranes were incubated with primary antibodies against SHMT1 (1:1000, D3B3J, CST), cyclinB (1:1000, 4138S, CST), CDC20 (1:1000, 4823S, CST), β‐actin (1:1000, A00702, GenScript), HOXD8 (1:1000, sc‐515357, SantaCruz), p21 (1:1000, A1483, ABclonal), γ‐H2AX (1:500, CSB‐PA010097OA139phHU, CUSABIO), p‐CDK1 (1:1000, CSB‐PA000492, CUSABIO), p‐CHK1 (1:1000, CSB‐PA006769, CUSABIO), p‐CDC25 (1:1000, CSB‐PA080107, CUSABIO), cyclinE (1:1000, sc‐377100, SANTA), and cyclinD (1:1000, WL01435a, WanleiBio) overnight at 4°C with shaking. .. Then, the membranes were washed with Tris Buffered Saline with Tween20 (TBST) and incubated with secondary antibody conjugated with HRP (1:5000, GenScript) for 1 h at room temperature.



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